PDHA1

Gene Symbol PDHA1
Entrez Gene 5160
Alt Symbol PDHA, PDHAD, PDHCE1A, PHE1A
Species Human
Gene Type protein-coding
Description pyruvate dehydrogenase (lipoamide) alpha 1
Other Description PDHE1-A type I|pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial|pyruvate dehydrogenase complex, E1-alpha polypeptide 1
Swissprots Q53H41 B7Z3T7 A5YVE9 Q9NP12 Q9UBJ8 Q5JPT8 Q9UNG5 Q9UBU0 P08559 Q9UNG4 B7Z3X5 B2R5P7
Accessions AAA31853 AAA60051 AAB32351 AAB39723 AAB39724 AAB39725 AAB39726 AAB39727 AAB39728 AAB39729 AAB39730 AAD16047 AAD23841 AAD23842 AAD23843 AAD23844 AAD23845 AAD23846 AAD23847 AAD23848 AAD23849 AAD23850 AAD23851 AAD23852 AAD23853 AAD23854 AAD23855 AAD23856 AAD23857 AAD23858 AAD23859 AAD23860 AAD23861 AAD23862 AAD23863 AAD23864 AAD23866 AAD23867 AAD23868 AAD23869 AAD23870 AAD23871 AAD23872 AAD23873 AAD23874 AAD23875 AAD23876 BAA14121 EAW98960 EAW98961 P08559 AK129887 AK222740 BAD96460 AK222988 BAD96708 AK293250 BAH11476 AK296341 BAH12323 AK296457 BAH12361 AK312263 BAG35194 AW204705 BC002406 AAH02406 BU170389 DA075402 DC361191 DQ891865 ABM82791 DQ895054 ABM85980 DQ895057 ABM85983 EF576990 ABQ58815 EF590116 EF590117 ABQ59099 J03503 AAA60055 J03575 AAA60050 L13318 AAA60227 L48690 AAB59581 M24848 AAA36533 R49470 S89908 AAB21863 X52709 CAA36933 X52710 CAA36934 XM_011545531 XP_011543833 XM_011545532 XP_011543834 NM_000284 NP_000275 NM_001173454 NP_001166925 NM_001173455 NP_001166926 NM_001173456 NP_001166927
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. {ECO:0000269|PubMed:19081061, ECO:0000269|PubMed:7782287}.
Subcellular Location Mitochondrion matrix.
Tissue Specificity Ubiquitous.
Top Pathways Glycolysis / Gluconeogenesis, Citrate cycle (TCA cycle), Central carbon metabolism in cancer, Glucagon signaling pathway, Pyruvate metabolism

Pyruvate Dehydrogenase Antibody - 2784 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using Pyruvate Dehydrogenase Antibody.

Pyruvate Dehydrogenase (C54G1) Rabbit mAb - 3205 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C54G1
Western blot analysis of extracts from various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.

PDH-E1α (D-6) - sc-377092 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PDH-E1α (D-6): sc-377092. Western blot analysis of PDH-E1α expression in EOC 20 (A), H4 (B) and IMR-32 (C) whole cell lysates.

PDH-E1α (H-131) - sc-292543 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PDH-E1α (H-131): sc-292543. Western blot analysis of PDH-E1α expression in Hep G2 (A) and K-562 (B) whole cell lysates and rat brain (C) and mouse brain (D) tissue extracts.

PDH-E1α (E-23) - sc-133898 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
PDH-E1α (E-23): sc-133898. Western blot analysis of PDH-E1α expression in rat brain tissue extract.

Anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) antibody [EPR12200] - ab177461 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12200
All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit (phospho S293) antibody [EPR12200] (ab177461) at 1/1000 dilutionLane 1 : Immunoprecipitation pellet from 293T cells lysate at 10 µgLane 2 : 1X PBS (negative control)SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody - ab67592 from Abcam

Prices $390.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilution + Human liver tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody - ab92695 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab92695) at 1 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody - ab110474 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab110474) at 1/500 dilutionLane 1 : Extracts from Jurkat cellsLane 2 : Extracts from HepG2 cellsLane 3 : Extracts from A549 cellsLane 4 : Extracts from Jurkat cells, treated with the synthesized peptideLysates/proteins at 30 µg per lane.

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody - ab151272 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab151272)diluted 1:100.

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [2C10] - ab119078 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C10
All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [2C10] (ab119078) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY Pyruvate Dehydrogenase E1-alpha subunitLysates/proteins at 5 µg per lane.

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [9H9AF5] (Alexa Fluor® 647) - ab197653 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9H9AF5
ab197653 staining Pyruvate Dehydrogenase E1-alpha subunit in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab197653 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [EPR11098] - ab168379 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11098
All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [EPR11098] (ab168379) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : A549 lysateLane 3 : Jurkat lysateLane 4 : HepG2 lysateLane 5 : HeLa lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [EPR11099] - ab155096 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11099
Overlay histogram showing HeLa cells stained with ab155096 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab155096, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293) - LS-C126204 from LifeSpan Bioscience

Prices $515.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293, Biotin) - LS-C141918 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293, DY650) - LS-C141919 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293, DY488) - LS-C141920 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293, HRP) - LS-C141921 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293, DY550) - LS-C141922 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser232) - LS-C163659 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Dot blot of anti-mPDHE1A-S232 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Anti-PDHA1 / PDH E1 Alpha Antibody (N-Terminus) - LS-C81158 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.

Anti-PDHA1 / PDH E1 Alpha Antibody (aa235-264, AP) - LS-C252525 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PDHA1 / PDH E1 Alpha Antibody (aa235-264, APC) - LS-C252526 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PDHA1 / PDH E1 Alpha Antibody (aa235-264, Biotin) - LS-C252527 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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