Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody

• Supplier: Abcam
• Catalog: ab67592
• Prices: $390.00
• Sizes: 50 µg
• Host: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: Full length PDHA1 protein (Human)
• Description: Mouse Polyclonal
• Gene: PDHA1
• Conjugate: Unconjugated

Product images

Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilution + Human liver tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilutionLane 1 : Pyruvate Dehydrogenase E1-alpha subunit transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

Immunohistochemical analysis of formalin fixed and paraffin embedded human stomach tissue labelled with ab67592 at 1.5µg/ml.

ICC/IF image of ab67592 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67592, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Hexokinase 2 is a key mediator of aerobic glycolysis and promotes tumor growth in - Hexokinase 2 is a key mediator of aerobic glycolysis and promotes tumor growth in

Wolf A, Agnihotri S, Micallef J, Mukherjee J, Sabha N, Cairns R, Hawkins C, Guha A. J Exp Med. 2011 Feb 14;208(2):313-26.

Nonproteasomal targets of the proteasome inhibitors bortezomib and carfilzomib: a - Nonproteasomal targets of the proteasome inhibitors bortezomib and carfilzomib: a

Arastu-Kapur S, Anderl JL, Kraus M, Parlati F, Shenk KD, Lee SJ, Muchamuel T, Bennett MK, Driessen C, Ball AJ, Kirk CJ. Clin Cancer Res. 2011 May 1;17(9):2734-43.

Contribution of impaired myocardial insulin signaling to mitochondrial - Contribution of impaired myocardial insulin signaling to mitochondrial

Boudina S, Bugger H, Sena S, O'Neill BT, Zaha VG, Ilkun O, Wright JJ, Mazumder PK, Palfreyman E, Tidwell TJ, Theobald H, Khalimonchuk O, Wayment B, Sheng X, Rodnick KJ, Centini R, Chen D, Litwin SE, Weimer BE, Abel ED. Circulation. 2009 Mar 10;119(9):1272-83.