All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab110474) at 1/500 dilutionLane 1 : Extracts from Jurkat cellsLane 2 : Extracts from HepG2 cellsLane 3 : Extracts from A549 cellsLane 4 : Extracts from Jurkat cells, treated with the synthesized peptideLysates/proteins at 30 µg per lane.
ab110474, at a 1/50 dilution, staining Pyruvate Dehydrogenase E1-alpha subunit in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the synthesized peptide.