All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [EPR11098] (ab168379) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : A549 lysateLane 3 : Jurkat lysateLane 4 : HepG2 lysateLane 5 : HeLa lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Pyruvate Dehydrogenase E1-alpha subunit with ab168379 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Pyruvate Dehydrogenase E1-alpha subunit with ab168379 at 1/100 dilution.
Immunofluorescent analysis of HepG2 cells labeling Pyruvate Dehydrogenase E1-alpha subunit with ab168379 at 1/100 dilution.
Flow cytometric analysis of permeabilized Jurkat cells labeling Pyruvate Dehydrogenase E1-alpha subunit (red) with ab168379 at 1/10 dilution, or a rabbit IgG (negative) (green).
Detection of Pyruvate Dehydrogenase E1-alpha subunit by Western Blot of Immunprecipitate. 293T cell lysate immunoprecipitated using ab168379 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.