Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [EPR11099]

• Supplier: Abcam
• Catalog: ab155096
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR11099
• Applications: WB IHC-P ICC/IF ICC/IF IP FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide, corresponding to residues in Human Pyruvate Dehydrogenase E1-alpha subunit (UniProt: P08559)
• Description: Rabbit Monoclonal
• Gene: PDHA1
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab155096 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab155096, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody [EPR11099] (ab155096) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Pyruvate Dehydrogenase E1-alpha subunit with ab155096 at 1/50 dilution.

Immunofluorescent analysis of HeLa cells labeling Pyruvate Dehydrogenase E1-alpha subunit with ab155096 at 1/250 dilution.