All lanes : Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (ab172617) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : Jurkat cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.
Immunofluorescent analysis of HepG2 cells labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab172617 at 1/10 dilution.
Flow cytometric analysis of permeabilized Jurkat cells labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).