Anti-RAB10 antibody [4E2]
| Name | Anti-RAB10 antibody [4E2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab104859 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 4E2 |
| Applications | ICC/IF ICC/IF WB ELISA FC |
| Species Reactivities | Mouse, Human |
| Antigen | Purified recombinant fragment of Human RAB10 expressed in E |
| Description | Mouse Monoclonal |
| Gene | RAB10 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-RAB10 antibody [4E2] (ab104859) at 1/500 dilutionLane 1 : Hela cell lysateLane 2 : NIH/3T3 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/19237_RAB10-Primary-antibodies-ab104859-1.jpg)
All lanes : Anti-RAB10 antibody [4E2] (ab104859) at 1/500 dilutionLane 1 : Hela cell lysateLane 2 : NIH/3T3 cell lysate
Immunofluorescence analysis of LOVO cells using ab104859 (green). Blue: DRAQ5 fluorescent DNA dye.
![Overlay histogram showing HeLa cells stained with ab104859 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104859, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91653, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/19239_RAB10-Primary-antibodies-ab104859-3.jpg)
Overlay histogram showing HeLa cells stained with ab104859 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104859, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91653, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.