All lanes : Anti-RAB11-FIP2 antibody [EPR12294] (ab174313) at 1/1000 dilutionLane 1 : 293T cell lysatesLane 2 : HeLa cell lysatesLysates/proteins at 10 µg per lane.
Flow cytometric analysis of permeabilized HeLa cells using ab174313 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Western blot analysis of immunoprecipitation pellet from 293T cell lysate (lane 1) or 1XPBS (negative control) (lane 2) using ab174313 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.