All lanes : Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504) at 1/10000 dilutionLane 1 : 293 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded, Human brain tissue labeling RAB11-FIP2 with ab180504 at a 1/250 dilution. Counter stained with hematoxylin.
Immunofluorescence analysis of, paraformaldehyde-fixed, 293 cells labeling RAB11-FIP2 with ab180504 at a 1/100 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/250. In blue DAPI staining.