Western blot analysis on immunoprecipitation pellet from (1) fetal brain lysate or (2) 1X PBS (negative control) using ab175925 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of Jurkat cells labeling RAB11B with ab175925 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
All lanes : Anti-RAB11B antibody [EPR12725] (ab175925) at 1/1000 dilutionLane 1 : Fetal brain lysateLane 2 : 293T cell lysateLane 3 : U87-MG cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.