All lanes : Anti-RAB13 antibody [EPR14109(B)] (ab180936) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryLane 1 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutionLane 2 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 10 µg
Lysate from HeLa cells was immunoprecipitated with ab180936 at a 1/70 dilution. For the subsequent blot, ab180936 used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. Second land is the negative control.