Anti-RAB27A antibody

Name	Anti-RAB27A antibody
Supplier	Abcam
Catalog	ab55667
Prices	$398.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Applications	WB IHC-P IP IHC-F ICC/IF ICC/IF
Species Reactivities	Mouse, Rat, Human, Rabbit
Antigen	Recombinant fragment: YCENPDIVLC GNKSDLEDQR VVKEEEAIAL AEKYGIPYFE TSAANGTNIS QAIEMLLDLI MKRMERCVDK SWIPEGVVRS NGHASTDQLS EEKEKGACGC , corresponding to amino acids 122-222 of Human RAB27A Run BLAST with Run BLAST with
Description	Mouse Monoclonal
Gene	RAB27A
Conjugate	Unconjugated
Supplier Page	Shop

Product images

RAB27A antibody (ab55667) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma. Heat induced antigen retrieval with Citrate buffer pH 6 has been performed prior to immunostaining.

ab55667 staining cow Retinal Pigment Epithelium (RPE) cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 20 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-mouse antibody was used as the secondary. Rab27a, which localizes to the apical site of the RPE cells, is labeled with red fluorescence. Nuclei were counterstained with DAPI (blue).See Abreview

All lanes : Anti-RAB27A antibody (ab55667) at 1/200 dilutionLane 1 : whole cell lysate of HEK 293 transfected with empty pCDNA3 vectorLane 2 : whole cell lysate of HEK 293 cells expressing Rab27a-GFPLysates/proteins at 20 µg per lane.SecondaryHRP conjugated goat anti-mousedeveloped using the ECL technique

$ab55667 (undiluted) was incubated with whole cell lysate of HEK 293 cells transfected with Rab27a-GFP and a Protein G matrix for 12 hours at 4°C to achieve immunoprecipitation. 20µg of lysate were present in the input.ab55667 (diluted 1/200) was also used in the western blotting step.Lane 1. lysate of HEK 293 cells expressing Rab27a-GFP Lane 2. IP with ab55667Lane 3. Not bound fractionSee Abreview$ ab55667 (undiluted) was incubated with whole cell lysate of HEK 293 cells transfected with Rab27a-GFP and a Protein G matrix for 12 hours at 4°C to achieve immunoprecipitation. 20µg of lysate were present in the input.ab55667 (diluted 1/200) was also used in the western blotting step.Lane 1. lysate of HEK 293 cells expressing Rab27a-GFP Lane 2. IP with ab55667Lane 3. Not bound fractionSee Abreview

Product References

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Suppression of antifolate resistance by targeting the myosin Va trafficking - Suppression of antifolate resistance by targeting the myosin Va trafficking

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Differential expression of Rab27A/B correlates with clinical outcome in - Differential expression of Rab27A/B correlates with clinical outcome in

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EPI64 protein functions as a physiological GTPase-activating protein for Rab27 - EPI64 protein functions as a physiological GTPase-activating protein for Rab27

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Distinct role of rab27a in granule movement at the plasma membrane and in the - Distinct role of rab27a in granule movement at the plasma membrane and in the

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