ab108983 staining RAB27A in Rhesus monkey fetal kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at 37°C. Samples were incubated with primary antibody (1/100 in 1% FCS/PBS) for 1 hour at 37°C. An Alexa Fluor®568-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview
All lanes : Anti-RAB27A antibody [EPR3021] (ab108983) at 1/1000 dilutionLane 1 : HeLa cell Lysate.Lane 2 : MCF-7 cell Lysate.Lane 3 : HL-60 cell Lysate.Lysates/proteins at 10 µg per lane.
Immunohistochemical staining of paraffin-embedded human tonsil tissue using anti- RAB27A (ab108983).