ChIP analysis of transcription factor binding to the Interferon β promoter before (A) and six hours after (B) Sendai virus infection of HeLa cells. Antibodies tested included NFκB p65 (A): sc-109, ATF-2 (N-96): sc-6233, GCN5 (N-18): sc-6303, Pol II (H-224): sc-9001, TFIID (TBP)(SI-1): sc-273 and CBP (A-22): sc-369. Data kindly provided by G. Mosialos.
GCN5 (N-18): sc-6303. Western blot analysis of GCN5 expression in K-562 (A), HeLa (B), MCF7 (C), NIH/3T3 (D) and UV treated HeLa (E) nuclear extracts.
GCN5 (N-18): sc-6303. Western blot analysis of GCN5 expression in non-transfected: sc-117752 (A) and human GCN5 transfected: sc-115311 (B) 293T whole cell lysates.
GCN5 (N-18): sc-6303. Western blot analysis of GCN5 expression in K-562 whole cell lysate.
GCN5 (N-18): sc-6303. Western blot analysis of GCN5 expression in non-transfected: sc-117752 (A) and human GCN5 transfected: sc-111723 (B) 293T whole cell lysates.
GCN5 (N-18): sc-6303. Western blot analysis of GCN5 expression in non-transfected: sc-117752 (A) and human GCN5 transfected: sc-115465 (B) 293T whole cell lysates.
GCN5 (N-18): sc-6303. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.