Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

• Supplier: Abcam
• Catalog: ab109534
• Prices: $388.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5438
• Applications: WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Rab5 aa 150-250
• Description: Rabbit Monoclonal
• Gene: RAB5A
• Conjugate: Unconjugated

Product images

All lanes : purified at 1/5000 dilutionLane 1 : Mouse brainLane 2 : Rat brainLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/5000 dilution (purified)Lane 1 : MCF7 cell lysateLane 2 : HEK293 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human pancreas with purified ab109534 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/1000 dilution (unpurified)Lane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 10 µg per lane.

Immunohistochemical staining of Rab5 in paraffin embedded human brain tissue with unpurified ab109534, at 1/100 dilution.