Anti-Rab5 antibody - C-terminal (ab175877) at 1/1000 dilution + HL60 cell lysate at 35 µg
Immunofluorescent analysis of paraffin-embedded Human brain tissue labeling Rab5 with ab175877 at 0.025 mg/ml followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Flow cytometric analysis of HL60 cells labeling Rab5 with ab175877 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human brain tissue labeling Rab5 with ab175877 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.