Immunohistochemical analysis of formalin fixed, paraffin embedded Human prostate tissue labeling Rab5 with ab190215 at 1/50 dilution.
Immunofluorescence on paraffin-embedded Human brain tissue labeling Rab5 with ab190215 at 25 μg/ml, followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.
Flow cytometry of HL60 cells (right histogram) compared to a negative control cell (left histogram), labeling Rab5 with ab190215 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-Rab5 antibody - C-terminal (ab190215) at 1/1000 dilution + HL60 cell line lysate at 35 µg