All lanes : Anti-RAB7 antibody (ab187868) at 1/1000 dilutionLane 1 : L6 (Rat skeletal muscle cell line) cell lysateLane 2 : L-929 (Mouse connective tissue fibroblast cells) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
RAB7 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate with ab187868 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab187868 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: HeLa cell lysate. Lane 2: PBS instead of HeLa cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling RAB7 with ab187868 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human breast carcinoma tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling RAB7 with ab187868 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.