ab137029 staining RAB7 in Human HepaRG cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% milk for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in 1% milk) for 30 minutes. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.See Abreview
All lanes : Anti-RAB7 antibody [EPR7589] (ab137029) at 1/1000 dilutionLane 1 : A375 cell lysateLane 2 : A431 cell lysateLane 3 : U87 MG cell lysateLane 4 : HT 1080 cell lysateLane 5 : L929 cell lysateLane 6 : NIH 3T3 cell lysateLane 7 : Raw 264.7 cell lysateLane 8 : C2C12 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP conjugated Goat anti Rabbit IgG at 1/2000 dilution
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling RAB7 with ab137029 at 1/50 dilution.