All lanes : Anti-RAB8A antibody [EPR14873] - C-terminal (ab188574) at 1/1000 dilutionLane 1 : C6 lysateLane 2 : RAW264.7 lysateLane 3 : PC-12 lysateLane 4 : NIH3T3 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-RAB8A antibody [EPR14873] - C-terminal (ab188574) at 1/1000 dilution + HCT-116 lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
All lanes : Anti-RAB8A antibody [EPR14873] - C-terminal (ab188574) at 1/10000 dilutionLane 1 : HepG2 lysateLane 2 : HeLa lysateLane 3 : 293T lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow Cytometrical analysis of HeLa cells labeling RAB8A with ab188574 at 1/260 (pink) compared to a Isotype control (green). FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labelling RAB8A with ab188574 at 1/500 followed by Goat anti rabbit IgG (Alexa Fluor®488) and DAPI staining.
Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of colon tissue labelling RAB8A with ab188574 at 1/500. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling RAB8A with ab188574 at 1/500. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counter stained with Hematoxylin.