Immunofluorescent analysis of U2OS cells fixed in 3% PFA for 10 mins and permeabilised with methanol for 2 mins, then irradiated with 3Gy of IR for 1.5h labeling RAD18 with ab188235 at 1/600 dilution. Rad18 (red) ; DAPI (blue).
Immunofluorescent analysis of RAD18 expression in U2OS control cells and RAD18 siRNA treated U2OS cells, using ab188235 at 1/2000 dilution.
Immunofluorescent analysis U2OS cells fixed in 3% PFA 10 mins and permeabilised with methanol 2 mins irradiated with 3Gy IR for 1.5h labeling RAD18 with ab188235 (1), DNA (2) and merged (3)
Western blot analysis (using ab188235) of immunoprecipitation from U2OS cell lysates(lysated in 0.5% NP40)Lane 1: InputLane 2: immunoprecipitate with control serum+protein A sepharose.Lane 3: immunoprecipitate with ab188235+ protein A sepharose.
All lanes : Anti-RAD18 antibody (ab188235) at 1/2000 dilutionLane 1 : Control U2OS cell lysateLane 2 : RAD18 si treated U2OS cell lysateLane 3 : NIH-3T3 cell lysateLysates/proteins at 40 µg per lane.