RAD18

Gene Symbol RAD18
Entrez Gene 56852
Alt Symbol RNF73
Species Human
Gene Type protein-coding
Description RAD18 E3 ubiquitin protein ligase
Other Description E3 ubiquitin-protein ligase RAD18|RAD18 homolog|RAD18, S. cerevisiae, homolog|RING finger protein 73|hHR18|hRAD18|postreplication repair protein RAD18|postreplication repair protein hRAD18p
Swissprots Q9NRT6 Q58F55 Q9NS91
Accessions AAX44049 EAW63948 EAW63949 Q9NS91 AB035274 BAA99284 AF169796 AAF80856 AK023075 BAB14392 AK123966 AK222771 BAD96491 AY004333 AAF86618 BC001302 AAH01302 DQ891197 ABM82123 DQ894380 ABM85306 DW433240 NM_020165 NP_064550
Function E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap- filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono- ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. {ECO:0000269|PubMed:17108083, ECO:0000269|PubMed:21659603}.
Subcellular Location Nucleus {ECO:0000250}.

Phospho-Rad18 (Ser403) Antibody - 8393 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 or HeLa cells, untreated or UV-treated, using Phospho-Rad18 (Ser403) Antibody (upper) or a total Rad18 antibody (lower).

Rad18 (D2B8) XP ® Rabbit mAb - 9040 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2B8
Western blot analysis of extracts from various cell lines using Rad18 (D2B8) XP ® Rabbit mAb.

Rad18 (H-77) - sc-98946 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rad18 (H-77): sc-98946. Western blot analysis of Rad18 expression in HeLa (A), HEL 92.1.7 (B), K-562 (C), MCF7 (D) and HL-60 (E) nuclear extracts.

Rad18 (V-16) - sc-27077 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rad18 (V-16): sc-27077. Western blot analysis of Rad18 expression in HeLa (A), UV-treated HeLa (B) and K-562 (C) nuclear extracts.

Rad18 (79B1048) - sc-52949 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Rad18 (79B1048): sc-52949. Western blot analysis of Rad18 expression in HeLa (A), UV-treated HeLa (B), HL-60 (C), MCF7 (D), K-562 (E) and HEL 92.1.7 (F) nuclear extracts.

Rad18 (4H237) - sc-71952 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Rad18 (4H237): sc-71952. Western blot analysis of Rad18 expression in HeLa (A), UV-treated HeLa (B), HL-60 (C), MCF7 (D), K-562 (E) and HEL 92.1.7 (F) nuclear extracts.

Anti-RAD18 (phospho S403) antibody [EPR8892] - ab138517 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8892
All lanes : Anti-RAD18 (phospho S403) antibody [EPR8892] (ab138517) at 1/1000 dilutionLane 1 : HeLa cell lysate untreated Lane 2 : HeLa cell lysate treated with Calyculin ALysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-RAD18 antibody - ab79763 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Indirect immunofluorescence staining of GM637 cells . Note staining of nuclei.

Anti-RAD18 antibody - ab188235 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of U2OS cells fixed in 3% PFA for 10 mins and permeabilised with methanol for 2 mins, then irradiated with 3Gy of IR for 1.5h labeling RAD18 with ab188235 at 1/600 dilution. Rad18 (red) ; DAPI (blue).

Anti-RAD18 antibody - ab186835 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186835. Blue DAPI for nuclear staining.

Anti-RAD18 antibody - ab97670 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-RAD18 antibody (ab97670) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-RAD18 antibody - ab138302 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-RAD18 antibody (ab138302) at 1/500 dilution + COLO205 cell lysate at 30 µg

Anti-RAD18 antibody - ab99334 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab99334 at 3µg/ml detecting RAD18 in HeLa whole cell lysate by IP. Lane 1: ab99334 IP at 3µg/mg of lysate Lane 2: IP with an antibody which recognizes an downstream epitope of RAD18 Lane 3: IP control IgG In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Subsequent WB detection was performed using an antibody that recognises a downstream epitope of RAD18. Detection: Chemiluminescence with an exposure time of 3 seconds. Two bands represent, but one or both likely represent a post-translationally modified form.

Anti-RAD18 antibody [79B1048] - ab12007 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 79B1048

Anti-RAD18 antibody [EPR8942] - ab157463 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8942
All lanes : Anti-RAD18 antibody [EPR8942] (ab157463) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : HCT-116 cell lysateLane 3 : K562 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-RAD18 antibody - ChIP Grade - ab57447 from Abcam

Prices $392.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
RAD18 antibody (ab57447) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.

Anti-RAD18 Antibody (aa175-225) - LS-C288149 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RAD18 Antibody (aa445-495) - LS-C288150 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RAD18 Antibody (aa301-350) - LS-C291326 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RAD18 Antibody (aa332-431, Azide-free) - LS-C328452 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-RAD18 Antibody - LS-C341600 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RAD18 Antibody (aa402-414, clone 79B1048.1) - LS-C542 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 79B1048.1
The anti-Rad18 mAb was diluted at 2 ug/ml and tested against 20 ug of HL-60 cell lysate.

Anti-RAD18 Antibody (aa251-300) - LS-C116231 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RAD18 antibody LS-C116231 Western blot of COLO205 cell lysate.

Anti-RAD18 Antibody (aa197-246) - LS-C80558 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-RAD18 Antibody (aa1-259) - LS-C186340 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded Cal27 Xenograft using RAD18 antibody at 1:100 dilution.
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