Anti-Rad21 antibody [52A311]

• Supplier: Abcam
• Catalog: ab153765
• Prices: $376.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 52A311
• Applications: IHC-P WB
• Species Reactivities: Human
• Antigen: Synthetic peptide (proprietary-tag) corresponding to Human Rad21 aa 250-650
• Description: Mouse Monoclonal
• Gene: RAD21
• Conjugate: Unconjugated

Product images

All lanes : Anti-Rad21 antibody [52A311] (ab153765) at 1 µg/mlLane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear ExtractLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilutionPerformed under reducing conditions.

developed using the ECL techniquePerformed under reducing conditions.

IHC image of Rad21 staining in human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab153765, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.