RAD21

Gene Symbol RAD21
Entrez Gene 5885
Alt Symbol CDLS4, HR21, HRAD21, MCD1, NXP1, SCC1, hHR21
Species Human
Gene Type protein-coding
Description RAD21 homolog (S. pombe)
Other Description NXP-1|SCC1 homolog|double-strand-break repair protein rad21 homolog|nuclear matrix protein 1|protein involved in DNA double-strand break repair|sister chromatid cohesion 1
Swissprots O60216 Q99568 Q15001 A8K0E0
Accessions AAT70725 EAW91963 EAW91964 EAW91965 O60216 AK097915 BAG53550 AK098521 AK125620 AK289505 BAF82194 BC001229 BC050381 AAH50381 D38551 BAA07554 DA328186 X98294 CAA66940 NM_006265 NP_006256
Function Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. {ECO:0000269|PubMed:11875078, ECO:0000269|PubMed:12417729}.
Subcellular Location Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis.
Top Pathways Cell cycle

RAD21 (D213) Antibody - 4321 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using RAD21 (D213) Antibody.

RAD21 (D5Y8S) Rabbit mAb - 12673 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5Y8S
Western blot analysis of extracts from various cell lines using RAD21 (D5Y8S) Rabbit mAb.

RAD21 (L611) Antibody - 4449 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using RAD21 (L611) Antibody.

Rad21 (B-2) - sc-271601 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rad21 (B-2): sc-271601. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

Rad21 (E-19) - sc-54323 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rad21 (E-19): sc-54323. Western blot analysis of Rad21 expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.

Rad21 (H-12) - sc-166973 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rad21 (H-12): sc-166973. Western blot analysis of Rad21 expression in HeLa (A) and MOLT-4 (B) nuclear extracts.

Rad21 (H-210) - sc-98784 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rad21 (H-210): sc-98784. Immunofluorescence staining of formalin-fixed HepG2 cells showing nuclear localization.

Rad21 (Q-20) - sc-54325 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rad21 (Q-20): sc-54325. Western blot analysis of Rad21 expression in HeLa (A) and MOLT-4 (B) nuclear extracts and HEK293 whole cell lysate (C).

Anti-Rad21 antibody - ab154769 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad21 antibody (ab154769) at 1/1000 dilutionLane 1 : K562 whole cell lysateLane 2 : THP1 whole cell lysateLane 3 : HL60 whole cell lysateLane 4 : NCI-H929 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Rad21 antibody - ab55892 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Rad21 antibody [52A311] - ab153765 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 52A311
All lanes : Anti-Rad21 antibody [52A311] (ab153765) at 1 µg/mlLane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear ExtractLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilutionPerformed under reducing conditions.

Anti-Rad21 antibody (Agarose) - ab19205 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Rad21 antibody [EPR12647] - ab175215 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12647
Flow cytometric analysis of permeabilized K562 cells labeling Rad21 with ab175215 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Anti-RAD21 Antibody (C-Terminus) - LS-C46992 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RAD21 Antibody (N-Terminus) - LS-C79482 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-RAD21 Antibody (C-Terminus) - LS-C93569 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RAD21 Antibody (Agarose) - LS-C93570 from LifeSpan Bioscience

Prices $635.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RAD21 Antibody - LS-C131786 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RAD21 Antibody - LS-C131785 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RAD21 Antibody (C-Terminus, clone CM110-2C10, Biotin) - LS-C141126 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone CM110-2C10

Anti-RAD21 Antibody (C-Terminus, clone CM110-2C10, DY650) - LS-C141127 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone CM110-2C10

Anti-RAD21 Antibody (C-Terminus, clone CM110-2C10, HRP) - LS-C141129 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone CM110-2C10

Anti-RAD21 Antibody (Internal) - LS-C153438 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RAD21 Antibody (Internal) - LS-C144918 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RAD21 Antibody (C-Terminus, clone CM110-2C10, DY550) - LS-C141130 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone CM110-2C10
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