Anti-Rad53 antibody
| Name | Anti-Rad53 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab104232 |
| Prices | $388.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB |
| Species Reactivities | Yeast |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Saccharomyces cerevisiae Rad53 |
| Description | Rabbit Polyclonal |
| Gene | RAD53 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Rad53 antibody (ab104232) at 1/2000 dilutionLane 1 : Saccharomyces cerevisiae whole cell lysate from exponentially growing cells- TCA prep.Lane 2 : Saccharomyces cerevisiae whole cell lysate from exponentially growing cells treated with 20mg/ml phleomycin for 1 hour - TCA prepLane 3 : Saccharomyces cerevisiae whole cell lysate from exponentially growing cells- TCA prepLane 4 : Saccharomyces cerevisiae whole cell lysate from exponentially growing cells arrested in G2 phase with nocodazole- TCA prep.Lane 5 : Saccharomyces cerevisiae whole cell lysate from exponentially growing cells arrested in G2 phase with nocodazole and treated with 20mg/ml phleomycin- TCA prep.Lysates/proteins at 20 µg per lane.SecondaryAmersham anti-rabbit IgG conjugated to HRP at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Rad53 antibody (ab104232) at 1/2000 dilutionLane 1 : TCA preps from exponentially growing WT yeast cells.Lane 2 : TCA preps from exponentially growing WT yeast cells treated with Hydroxyurea (HU).Lane 3 : TCA preps from exponentially growing rad53delta yeast cells.Secondary1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Rad53 antibody (ab104232) at 1/1000 dilutionLane 1 : Alpha-factor arrested WT cells.Lane 2 : WT cells 20' after release in S-phase.Lane 3 : WT cells 40' after release in S-phase.Lane 4 : WT cells 60' after release in S-phase.Lane 5 : Alpha-factor arrested WT cells.Lane 6 : WT cells 20' after release in S-phase in 200mM HU.Lane 7 : WT cells 40' after release in S-phase in 200mM HU.Lane 8 : WT cells 60' after release in S-phase in 200mM HU.Lane 9 : Alpha-factor arrested rad53delta cells.Lane 10 : rad53delta cells 20' after release in S-phase.Lane 11 : rad53delta cells 40' after release in S-phase.Lane 12 : rad53delta cells 60' after release in S-phase.Lane 13 : Alpha-factor arrested rad53delta cells.Lane 14 : rad53delta cells 20' after release in S-phase in 200mM HU.Lane 15 : rad53delta cells 40' after release in S-phase in 200mM HU.Lane 16 : rad53delta cells 60' after release in S-phase in 200mM HU.SecondaryDonkey Anti-Rabbit IgG H&L (HRP) (ab16284) at 1/2000 dilutionde
developed using the ECL techniquePerformed under reducing conditions.
Product References
An RNA polymerase II-coupled function for histone H3K36 methylation in checkpoint - An RNA polymerase II-coupled function for histone H3K36 methylation in checkpoint
Jha DK, Strahl BD. Nat Commun. 2014 Jun 9;5:3965.
Site-specific phosphorylation of the DNA damage response mediator rad9 by - Site-specific phosphorylation of the DNA damage response mediator rad9 by
Abreu CM, Kumar R, Hamilton D, Dawdy AW, Creavin K, Eivers S, Finn K, Balsbaugh JL, O'Connor R, Kiely PA, Shabanowitz J, Hunt DF, Grenon M, Lowndes NF. PLoS Genet. 2013 Apr;9(4):e1003310.