RAD53

Gene Symbol RAD53
Entrez Gene 855950
Alt Symbol LSD1, MEC2, SPK1
Species Yeast
Gene Type protein-coding
Description serine/threonine/tyrosine protein kinase RAD53
Other Description -
Swissprots Q6B1S1 P22216 D6W3L5
Accessions DAA11281 P22216 NM_001183967 NP_015172
Function Controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints. Phosphorylates proteins on serine, threonine, and tyrosine. Prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. Seems to be involved in the phosphorylation of RPH1. {ECO:0000269|PubMed:10550056, ECO:0000269|PubMed:11809875, ECO:0000269|PubMed:15024067, ECO:0000269|PubMed:7958905, ECO:0000269|PubMed:8355715}.
Subcellular Location Nucleus {ECO:0000269|PubMed:8355715}.
Top Pathways Cell cycle - yeast, Meiosis - yeast

Rad53 (A-9) - sc-74427 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rad53 (A-9): sc-74427. Western blot analysis of Yeast recombinant Rad53 fusion protein.

Rad53 (B-6) - sc-74426 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rad53 (B-6): sc-74426. Western blot analysis of yeast recombinant Rad53 fusion protein.

Rad53 (y-300) - sc-20169 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rad53 (y-300): sc-20169. Western blot analysis of yeast recombinant Rad53 fusion protein.

Rad53 (yC-19) - sc-6749 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Rad53 (yN-19) - sc-6748 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Rad53 antibody - ab104232 from Abcam

Prices $388.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad53 antibody (ab104232) at 1/2000 dilutionLane 1 : Saccharomyces cerevisiae whole cell lysate from exponentially growing cells- TCA prep.Lane 2 : Saccharomyces cerevisiae whole cell lysate from exponentially growing cells treated with 20mg/ml phleomycin for 1 hour - TCA prepLane 3 : Saccharomyces cerevisiae whole cell lysate from exponentially growing cells- TCA prepLane 4 : Saccharomyces cerevisiae whole cell lysate from exponentially growing cells arrested in G2 phase with nocodazole- TCA prep.Lane 5 : Saccharomyces cerevisiae whole cell lysate from exponentially growing cells arrested in G2 phase with nocodazole and treated with 20mg/ml phleomycin- TCA prep.Lysates/proteins at 20 µg per lane.SecondaryAmersham anti-rabbit IgG conjugated to HRP at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Rad53 antibody - ab150018 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad53 antibody (ab150018) at 1/2000 dilutionLane 1 : TCA preps from wild-type Saccharomyces cerevisiae cellsLane 2 : TCA preps from wild-type Saccharomyces cerevisiae cells treated with Camptothecin for 2 hrs.Lane 3 : TCA preps from wild-type Saccharomyces cerevisiae cells treated with Camptothecin for 4 hrs.SecondaryAnti-rabbit at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Rad53 antibody [EL7.E1] - ab166859 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone EL7.E1
All lanes : Anti-Rad53 antibody [EL7.E1] (ab166859) at 1 µg/mlLane 1 : TCA preps from S. cerevisiae cellsLane 2 : TCA preps from S. cerevisiae cells treated with Hydroxyurea (HU)Lysates/proteins at 15 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-S. cerevisiae RAD53 Polyclonal antibody - DPABH-29283 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Anti-S. cerevisiae RAD53 Polyclonal antibody - DPABH-29298 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal
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