Anti-RAGE antibody
| Name | Anti-RAGE antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3611 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-F WB FC IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Rat RAGE aa 362-380 |
| Description | Rabbit Polyclonal |
| Gene | AGER |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab3611 used in IHC (frozen) in transgenic mouse retinas.
ab3611 at 1/200 staining mouse BV2 cells by ICC/IF. The cells were formaldehyde fixed, permeabilized and blocked with serum before incubation with the antibody for 1 hour. An Alexa Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary. The left hand panel shows unstimulated cells, the right hand panel LPS stimulated cells.See Abreview
Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse heart tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing RAGE ab3611 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing RAGE ab3611 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue . To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing RAGE ab3611 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Product References
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