Anti-RAGE antibody
| Name | Anti-RAGE antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54741 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB FC IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 23-405 of Human RAGE |
| Description | Mouse Monoclonal |
| Gene | AGER |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot against tagged recombinant protein immunogen using ab54741 RAGE antibody at 1ug/ml.
All lanes : Anti-RAGE antibody (ab54741) at 1 µg/mlLane 1 : 293T RAGE transfected cell lysateLane 2 : 293T non-transfected cell lysate
ab54741 staining human Jurkat T cells by Flow Cytometry. The cells were prepared in PBS with 0.2% BSA. The primary antibody diluted 1/100 and incubated with sample for 30 minutes at 0°C. The secondary antibody was Alexa Fluor® 488 conjugated goat polyclonal to mouse IgG, diluted 1/200. Specimen tube 001 is negative control.See Abreview
ab54741 (1 µg/ml) staining RAGE in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is membrane staining throughout the alveloli.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
MK615 decreases RAGE expression and inhibits TAGE-induced proliferation in - MK615 decreases RAGE expression and inhibits TAGE-induced proliferation in
Sakuraoka Y, Sawada T, Okada T, Shiraki T, Miura Y, Hiraishi K, Ohsawa T, Adachi M, Takino J, Takeuchi M, Kubota K. World J Gastroenterol. 2010 Nov 14;16(42):5334-41.
Irreversibly glycated LDL induce oxidative and inflammatory state in human - Irreversibly glycated LDL induce oxidative and inflammatory state in human
Toma L, Stancu CS, Botez GM, Sima AV, Simionescu M. Biochem Biophys Res Commun. 2009 Dec 18;390(3):877-82. doi: