Anti-RAGE antibody

• Supplier: Abcam
• Catalog: ab30381
• Prices: $394.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IHC-F
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human RAGE
• Description: Rabbit Polyclonal
• Gene: AGER
• Conjugate: Unconjugated

Product images

Anti-RAGE antibody (ab30381) at 1 µg/ml + Mouse Lung Whole Tissue Lysate at 20 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.

Immunofluorescent staining for RAGE in the mouse brain using ab30381. The antiboby stains processes of neurons in many brain areas, the above image shows staining in Caudate Putamen and Cortex. Please see accompanying abreview for additional information.See Abreview

ICC/IF image of ab30381 stained T24-83 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab30381 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Advanced glycation end products induce a prothrombotic phenotype in mice via - Advanced glycation end products induce a prothrombotic phenotype in mice via

Zhu W, Li W, Silverstein RL. Blood. 2012 Jun 21;119(25):6136-44.

Oxidative stress-associated rise of hepatic protein glycation increases - Oxidative stress-associated rise of hepatic protein glycation increases

Kuhla A, Hettwer C, Menger MD, Vollmar B. Lab Invest. 2010 Aug;90(8):1189-98.