Anti-RanGAP1 antibody

• Supplier: Abcam
• Catalog: ab4784
• Prices: $390.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Human, Xenopus
• Antigen: Synthetic peptide mixture: CHWSDMFTGRLRPEI (amino acids 82-96) and CPSPEKLVRMGPRRSA (amino acids 434-448) from Xenopus laevis Run BLAST with Run BLAST with
• Description: Rabbit Polyclonal
• Gene: RANGAP1
• Conjugate: Unconjugated

Product images

ab4784 staining RanGAP1 on skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) 

ab4784 at a 1/500 dilution staining approximately 90kDa RanGAP 1 in Xenopus egg extract (lane X, 50µg per lane) and HeLa total cell extract (lane Hu, 50µg per lane) by western blot (ECL, 1 minute exposure). NB: RanGAP 1 in the above blot is modified by the addition of the SUMO-modifier peptide, so runs at approximately 90 kDa. The smaller band visible in the Xenopus lane at approximately 60 kDa is likely to be a small amount of non-SUMOylated protein.ab4784 at a 1/500 dilution staining approximately 90kDa RanGAP 1 in Xenopus egg extract (lane X, 50µg per lane) and HeLa total cell extract (lane Hu, 50µg per lane) by western blot (ECL, 1 minute exposure). NB: RanGAP 1 in the above blot is modified by the addition of the SUMO-modifier peptide, so runs at approximately 90 kDa. The smaller band visible in the Xenopus lane at approximately 60 kDa&#

ab4784 (1/50) staining RanGap1 in mouse NIH-3T3 cells (green). Cells were fixed in methanol (-20oC/ 6 mins, followed by 3 washes in PBS) and DAPI was used as counterstain in order to highlight the nucleus (blue);

Product References

Tumor cell dependence on Ran-GTP-directed mitosis. - Tumor cell dependence on Ran-GTP-directed mitosis.

Xia F, Lee CW, Altieri DC. Cancer Res. 2008 Mar 15;68(6):1826-33.

Regulation of Wnt signaling by the nuclear pore complex. - Regulation of Wnt signaling by the nuclear pore complex.

Shitashige M, Satow R, Honda K, Ono M, Hirohashi S, Yamada T. Gastroenterology. 2008 Jun;134(7):1961-71, 1971.e1-4. doi:

An extended consensus motif enhances the specificity of substrate modification by - An extended consensus motif enhances the specificity of substrate modification by

Yang SH, Galanis A, Witty J, Sharrocks AD. EMBO J. 2006 Nov 1;25(21):5083-93. Epub 2006 Oct 12.