Anti-RANK antibody [9A725]
| Name | Anti-RANK antibody [9A725] |
|---|---|
| Supplier | Abcam |
| Catalog | ab12008 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 9A725 |
| Applications | WB ICC/IF ICC/IF FC IHC-P |
| Species Reactivities | Human |
| Antigen | Fusion protein, corresponding to amino acids 326-616 of Human RANK |
| Description | Mouse Monoclonal |
| Gene | TNFRSF11A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
RANK detection by Western blot. The analysis of RANK in 293 cells transfected with RANK cDNA. A single protein band of approximate mol. wt. of 97 kDa was detected by using anti-RANK at 2 ug/ml.
![Overlay histogram showing HepG2 cells stained with ab12008 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12008, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/20630_RANK-Primary-antibodies-ab12008-1.jpg){kind=icon}
Overlay histogram showing HepG2 cells stained with ab12008 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12008, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
ICC/IF image of ab12008 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12008, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Vps35 loss promotes hyperresorptive osteoclastogenesis and osteoporosis via - Vps35 loss promotes hyperresorptive osteoclastogenesis and osteoporosis via
Xia WF, Tang FL, Xiong L, Xiong S, Jung JU, Lee DH, Li XS, Feng X, Mei L, Xiong WC. J Cell Biol. 2013 Mar 18;200(6):821-37.
Differential expression of the RANKL/RANK/OPG system is associated with bone - Differential expression of the RANKL/RANK/OPG system is associated with bone
Peng X, Guo W, Ren T, Lou Z, Lu X, Zhang S, Lu Q, Sun Y. PLoS One. 2013;8(3):e58361.
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin - Functional expression of receptor activator of nuclear factor kappaB in Hodgkin
Fiumara P, Snell V, Li Y, Mukhopadhyay A, Younes M, Gillenwater AM, Cabanillas F, Aggarwal BB, Younes A. Blood. 2001 Nov 1;98(9):2784-90.