Anti-RANTES antibody

• Supplier: Abcam
• Catalog: ab9783
• Prices: $382.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: unknown
• Applications: ELISA ELISA WB
• Species Reactivities: Rat
• Antigen: Highly pure (>98%) recombinant rRANTES (rat RANTES)
• Description: Rabbit Polyclonal
• Gene: Ccl5
• Conjugate: Unconjugated

Product images

Indirect ELISA measuring RANTES levels using ab9783. Supernatants collected from rat cerebral cortical cells after various treatments were coated onto 96-well flat bottom plates and incubated overnight at 4°C. The plates were blocked with 1% BSA at 37°C for 45 min before incubation with primary antibody (1/1000) in bicarbonate buffer overnight at 4°C. An HRP-conjugated secondary IgG (1/1000) was added and incubated for 45 min at 37°C, in the dark. The reaction was developed by adding o-phenylene diamine H2O2 for 20 min. Optical density was measured at 450 nm.

Product References

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Tripathy D, Grammas P. Microvasc Res. 2009 May;77(3):289-96.

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Tripathy D, Grammas P. J Neuroinflammation. 2009 Mar 16;6:10.

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Sanchez A, Tripathy D, Grammas P. Neuropeptides. 2009 Aug;43(4):315-20.

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