All lanes : Anti-RAP1A + RAP1B antibody [EPR14814] (ab187659) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLane 3 : MOLT4 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-RAP1A + RAP1B antibody [EPR14814] (ab187659) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation analysis of A431 cell lysate labeling RAP1A + RAP1B with ab187659 at 1/50 dilution. Secondary staining with anti-Rabbit IgG (HRP), specific to the non-reduced form of IgGat 1/1500 dilution.