Anti-RASA1 antibody [B4F8]

• Supplier: Abcam
• Catalog: ab2922
• Prices: $385.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: B4F8
• Applications: ICC/IF ICC/IF IP WB
• Species Reactivities: Mouse, Rat, Bovine, Human, Primate
• Antigen: Recombinant full length protein corresponding to Human RASA1
• Description: Mouse Monoclonal
• Gene: RASA1
• Conjugate: Unconjugated

Product images

ab2922 labelling GAP (green) in the cytoplasm of A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

ab2922 labelling GAP (green) in the cytoplasm of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

ab2922 labelling GAP (green) in the cytoplasm of Hela cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

All lanes : Anti-RASA1 antibody [B4F8] (ab2922) at 1/200 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : Mouse brain cell lysateLysates/proteins at 25 µg per lane.Observed band size : 110 kDa (why is the actual band size different from the predicted?)

Product References

Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications - Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications

Settleman J, Narasimhan V, Foster LC, Weinberg RA. Cell. 1992 May 1;69(3):539-49.