RASA1

Gene Symbol RASA1
Entrez Gene 5921
Alt Symbol CM-AVM, CMAVM, GAP, PKWS, RASA, RASGAP, p120GAP, p120RASGAP
Species Human
Gene Type protein-coding
Description RAS p21 protein activator (GTPase activating protein) 1
Other Description ras GTPase-activating protein 1|triphosphatase-activating protein
Swissprots Q9UDI1 B4DTL2 Q68CU6 P20936 B2R6W3
Accessions EAW95931 EAW95932 EAW95933 P20936 AB209106 BAD92343 AK300263 BAG62024 AK300269 BAG62030 AK300406 BAG62136 AK312739 BAG35610 BC020761 AAH20761 BC033015 AAH33015 BC054891 AAH54891 BC070063 BC107870 BC131816 AAI31817 CF528258 CR749722 CAH18488 DA863496 M23379 AAA52517 M23612 AAA35865 XM_011543525 XP_011541827 XM_011543526 XP_011541828 XM_011543527 XP_011541829 NM_002890 NP_002881 NM_022650 NP_072179
Function Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1. {ECO:0000269|PubMed:11389730, ECO:0000269|PubMed:8360177}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:8360177}.
Tissue Specificity In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level). {ECO:0000269|PubMed:8360177}.
Top Pathways Axon guidance, MAPK signaling pathway

Ras GAP (171) - sc-425 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Ras GAP (171): sc-425. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.

Ras GAP (B4F8) - sc-63 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ras GAP (B4F8) PE: sc-63 PE. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal mouse IgG<sub>2a</sub>: sc-2867.

Ras GAP (D-18) - sc-32047 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Ras GAP (D-6) - sc-271436 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ras GAP (D-6): sc-271436. Western blot analysis of Ras GAP expression in A-431 whole cell lysate.

Ras GAP (P-16) - sc-32049 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-Ras GAP (pY460.19A) - sc-136481 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p-Ras GAP (pY460.19A): sc-136481. Western blot analysis of Ras GAP phosphorylation in untreated (A) and alkaline phosphatase-treated (B) RSV-3T3 cells.

Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] - ab109465 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2620(2)
All lanes : Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465) at 1/1000 dilutionLane 1 : 293T cell lysates, untreated Lane 2 : 293T cell lysates treated with PervanadateLysates/proteins at 10 µg per lane.SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution

Anti-RASA1 antibody - ab40807 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab40807 at a 1:100 dilution staining RASA1 in Human breast carcinoma tissue.

Anti-RASA1 antibody - ab18841 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RASA1 antibody - ab55663 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
GAP antibody (ab55663) used in immunohistochemistry at 6ug/ml on formalin fixed and paraffin embedded human small Intestine.

Anti-RASA1 antibody [1H2] - ab139301 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H2
All lanes : Anti-RASA1 antibody [1H2] (ab139301) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY GAP cDNALysates/proteins at 5 µg per lane.

Anti-RASA1 antibody [B4F8] - ab2922 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone B4F8
ab2922 labelling GAP (green) in the cytoplasm of A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

Anti-RASA1 antibody [EP536Y] - ab40677 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP536Y
Anti-RASA1 antibody [EP536Y] (ab40677) at 1/2000 dilution + Rat brain lysate at 10 µg

Anti-RASA1 antibody - C-terminal - ab171432 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-RASA1 antibody - C-terminal (ab171432) at 1/100 dilution + 293 cell line lysate at 35 µg

Anti-RASA1 Antibody (aa132-350) - LS-C38754 from LifeSpan Bioscience

Prices $475.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal

Anti-RASA1 / RASGAP Antibody (C-Terminus) - LS-C38757 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-RASA1 / RASGAP Antibody - LS-C38758 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-RASA1 Antibody - LS-C38759 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-RASA1 Antibody (aa171-448) - LS-C38760 from LifeSpan Bioscience

Prices $475.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal

Anti-RASA1 Antibody (aa1034-1047) - LS-C38761 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RASA1 Antibody (aa158-455) - LS-C38762 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RASA1 Antibody (clone 2C12) - LS-C133082 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C12
RASA1 monoclonal antibody (M01), clone 2C12 Western blot of RASA1 expression in IMR-32.

Anti-RASA1 Antibody (aa953-1002) - LS-C135002 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RASA1 antibody LS-C135002 Western blot of Mouse Brain lysate. Antibody concentration 1 ug/ml.

Anti-RASA1 Antibody - LS-C48306 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of RASA1 / RASGAP antibody LS-C48306.

Anti-RASA1 Antibody (aa966-993, AP) - LS-C239752 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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