Anti-RASSF1a antibody [3F3]
| Name | Anti-RASSF1a antibody [3F3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab23950 |
| Prices | $392.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 3F3 |
| Applications | ELISA WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant human RASSF1A purified from E |
| Description | Mouse Monoclonal |
| Gene | RASSF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-RASSF1a antibody [3F3] (ab23950) at 1/1000 dilutionLane 1 : Hela (H) cell extractLane 2 : Mouse brain (MB) extractLane 3 : Mouse liver (ML) extractLane 4 : Mouse kidney (MK) extractLysates/proteins at 20 µg per lane.SecondaryGoat anti-mouse HRP conjugatedeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/21003_ab23950_1.jpg)
All lanes : Anti-RASSF1a antibody [3F3] (ab23950) at 1/1000 dilutionLane 1 : Hela (H) cell extractLane 2 : Mouse brain (MB) extractLane 3 : Mouse liver (ML) extractLane 4 : Mouse kidney (MK) extractLysates/proteins at 20 µg per lane.SecondaryGoat anti-mouse HRP conjugatedeveloped using the ECL technique
A431 cells were labeled with anti-RASSF1a(clone 3F3) ab23950. Detection was done using a biotinylated secondary antibody and Texas-red conjugated streptavidin.
![All lanes : Anti-RASSF1a antibody [3F3] (ab23950) at 1/1000 dilutionLane 1 : HEK293 cellsLane 2 : A549 cellsLane 3 : HeLa cells](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/21005_ab23950_3.jpg)
All lanes : Anti-RASSF1a antibody [3F3] (ab23950) at 1/1000 dilutionLane 1 : HEK293 cellsLane 2 : A549 cellsLane 3 : HeLa cells
![Overlay histogram showing HeLA cells stained with ab23950 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab23950, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/21006_RASSF1a-Primary-antibodies-ab23950-1.jpg)
Overlay histogram showing HeLA cells stained with ab23950 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab23950, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Epidermal growth factor receptor (EGFR)-RAS signaling pathway in penile squamous - Epidermal growth factor receptor (EGFR)-RAS signaling pathway in penile squamous
Gou HF, Li X, Qiu M, Cheng K, Li LH, Dong H, Chen Y, Tang Y, Gao F, Zhao F, Men HT, Ge J, Su JM, Xu F, Bi F, Gao JJ, Liu JY. PLoS One. 2013 Apr 24;8(4):e62175.
Epigenetic silencing of RASSF1A deregulates cytoskeleton and promotes malignant - Epigenetic silencing of RASSF1A deregulates cytoskeleton and promotes malignant
Korah R, Healy JM, Kunstman JW, Fonseca AL, Ameri AH, Prasad ML, Carling T. Mol Cancer. 2013 Aug 5;12:87.
The intronic long noncoding RNA ANRASSF1 recruits PRC2 to the RASSF1A promoter, - The intronic long noncoding RNA ANRASSF1 recruits PRC2 to the RASSF1A promoter,
Beckedorff FC, Ayupe AC, Crocci-Souza R, Amaral MS, Nakaya HI, Soltys DT, Menck CF, Reis EM, Verjovski-Almeida S. PLoS Genet. 2013;9(8):e1003705.
RASSF1A inhibits estrogen receptor alpha expression and estrogen-independent - RASSF1A inhibits estrogen receptor alpha expression and estrogen-independent
Thaler S, Schmidt M, Schad A, Sleeman JP. Oncogene. 2012 Nov 22;31(47):4912-22.
The tumor suppressor RASSF1A prevents dephosphorylation of the mammalian - The tumor suppressor RASSF1A prevents dephosphorylation of the mammalian
Guo C, Zhang X, Pfeifer GP. J Biol Chem. 2011 Feb 25;286(8):6253-61.
RASSF1A mediates p21Cip1/Waf1-dependent cell cycle arrest and senescence through - RASSF1A mediates p21Cip1/Waf1-dependent cell cycle arrest and senescence through
Thaler S, Hahnel PS, Schad A, Dammann R, Schuler M. Cancer Res. 2009 Mar 1;69(5):1748-57.
AP-2alpha induces epigenetic silencing of tumor suppressive genes and - AP-2alpha induces epigenetic silencing of tumor suppressive genes and
Bennett KL, Romigh T, Eng C. PLoS One. 2009 Sep 9;4(9):e6931.
RASSF1A interacts with and activates the mitotic kinase Aurora-A. - RASSF1A interacts with and activates the mitotic kinase Aurora-A.
Liu L, Guo C, Dammann R, Tommasi S, Pfeifer GP. Oncogene. 2008 Oct 16;27(47):6175-86.
Id-1 promotes chromosomal instability through modification of APC/C activity - Id-1 promotes chromosomal instability through modification of APC/C activity
Wang X, Di K, Zhang X, Han HY, Wong YC, Leung SC, Ling MT. Oncogene. 2008 Jul 24;27(32):4456-66.
Hypermethylation of RASSF1A in human and rhesus placentas. - Hypermethylation of RASSF1A in human and rhesus placentas.
Chiu RW, Chim SS, Wong IH, Wong CS, Lee WS, To KF, Tong JH, Yuen RK, Shum AS, Chan JK, Chan LY, Yuen JW, Tong YK, Weier JF, Ferlatte C, Leung TN, Lau TK, Lo KW, Lo YM. Am J Pathol. 2007 Mar;170(3):941-50.