ab6545 staining Rb2 p130 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde in PBS at 4°C overnight, embedded in paraffin was and section a 7μm. Endogenous enzyme activity was quenched by 2% H2O2 in methanol (30 minutes). Sections were blocked wirh 2% BSA, 5% serum and 0.1% Trition X-100 in PBS. Samples were incubated with primary antibody (1/500 in blocking buffer). White arrow heads mark epithelial cells expressing high levels of protein in nuclear and perinuclear locations.
Immunohistochemical staining of mouse tissue using anti-pRb2 / p130 antiserum. The staining shows the location of pRb2 / p130 in developing mouse tissue. References for Anti-Rb2 p130 antibody (ab6545) This product has been referenced in: Herriges MJ et al. Long noncoding RNAs are spatially correlated with transcription factors and regulate lung development. Genes Dev 28:1363-79 (2014). Read more (PubMed: 24939938) » Cerezo A et al. Absence of Caveolin-1 Increases Proliferation and Anchorage-Independent Growth by a Rac-Dependent, Erk-Independent Mechanism. Mol Cell Biol : (2009). WB ; Mouse . Read more (PubMed: 19620284) » See all 3 Publications for this product Publishing research using ab6545 ? Please let us know so that we can cite the reference in this datasheet