Anti-RbBP5 antibody
| Name | Anti-RbBP5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab87190 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Rabbit, Horse, Bovine, Cat, Dog, Pig |
| Antigen | Synthetic peptide corresponding to a region within the internal sequence amino acids 432-481 (ASSEKKRQSS ADGSQPPKKK PKTTNIELQG VPNDEVHPLL GVKGDGKSKK) of Human RbBP5, NP_005048 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | RBBP5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-RbBP5 antibody (ab87190) at 1 µg/ml + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab87190 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87190, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) SHSY5Y cells at 5µg/ml