mouse brain tissue were subjected to SDS PAGE followed by western blot with 14821-1-AP(ZFYVE9 antibody) at dilution of 1:1000
IP Result of anti-ZFYVE9 (IP:14821-1-AP, 5ug; Detection:14821-1-AP 1:500) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of HepG2 cells, using ZFYVE9 antibody 14821-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
1X10^6 HepG2 cells were stained with 0.2ug ZFYVE9 antibody (14821-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.