ZFYVE9

Gene Symbol ZFYVE9
Entrez Gene 9372
Alt Symbol MADHIP, NSP, PPP1R173, SARA, SMADIP
Species Human
Gene Type protein-coding
Description zinc finger, FYVE domain containing 9
Other Description MAD, mothers against decapentaplegic homolog interacting protein, receptor activation anchor|MADH-interacting protein|novel serine protease|protein phosphatase 1, regulatory subunit 173|smad anchor for receptor activation|zinc finger FYVE domain-containing protein 9
Swissprots Q5T0F7 Q9Y5R7 O95405 Q9UNE1 Q5T0F6
Accessions CCQ43282 EAX06790 EAX06791 EAX06792 O95405 AB209125 BAD92362 AF104304 AAC99462 AF130419 AAD31694 AF130420 AAD31695 BC032680 AAH32680 DR005493 EU176568 ABW03369 XM_011542437 XP_011540739 NM_004799 NP_004790 NM_007324 NP_015563 NM_007323
Function Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization. {ECO:0000269|PubMed:15356634, ECO:0000269|PubMed:9865696}.
Subcellular Location Cytoplasm. Early endosome membrane.
Tissue Specificity Ubiquitous. In the brain found primarily in the cerebrovascular smooth muscle cells and reactive astrocytes.
Top Pathways TGF-beta signaling pathway, Endocytosis

Sara (D5X4F) Rabbit mAb - 13285 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5X4F
Western blot analysis of extracts from SH-SY5Y cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® Sara siRNA I (+) #13542, or SignalSilence ® Sara siRNA II #13553 (+), using Sara (D5X4F) Rabbit mAb (upper) or α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower). The Sara (D5X4F) Rabbit mAb confirms silencing of Sara expression, while the α-Actinin (D6F6) XP ® Rabbit mAb is used as a loading control.

SARA (F-9) - sc-133071 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SARA (F-9): sc-133071. Western blot analysis of SARA expression in HeLa (A) and Jurkat (B) whole cell lysates.

SARA (B-9) - sc-74493 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SARA (B-9): sc-74493. Western blot analysis of SARA expression in HeLa (A) and Caki-1 (B) whole cell lysates.

SARA (H-300) - sc-9135 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SARA (H-300): sc-9135. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.

SARA (N-20) - sc-8881 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SARA (N-20): sc-8881. Western blot analysis of SARA expression in HeLa whole cell lysate.

Anti-SARA antibody - ab168555 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-SARA antibody (ab168555) at 1 µg/ml + HeLa cell lysate at 50 µgSecondaryGoat anti-rabbit HRP conjugated antibody at 1/7500 dilution

Anti-SARA antibody [EPR5020] - ab124875 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5020
All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilutionLane 1 : Human heart lysateLane 2 : A673 cell lysateLane 3 : HT-1080 cell lysateLane 4 : BxPC-3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-ZFYVE9 / SARA Antibody (N-Terminus, clone EPR5020) - LS-C138665 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5020
IHC of paraffin-embedded human kidney tissue using anti-SARA antibody.

Anti-ZFYVE9 / SARA Antibody (aa1-50) - LS-C290421 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human SARA by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SARA antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SARA was also immunoprecipitated by rabbit anti-SARA antibody BL13545, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

ZFYVE9 Antibody - H00009372-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ZFYVE9 Antibody - H00009372-D01 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ZFYVE9 (601-615) antibody produced in rabbit - SAB1100744 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-ZFYVE9 (601-615) : <B>Cat. No. SAB1100744</B>: Immunofluorescence of HUVEC cells using ZFYVE9 (601-615) , Cat. No. SAB1100744 (red) at a 1:100 dilution, taken at 40x magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-ZFYVE9 antibody produced in rabbit - SAB1411651 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ZFYVE9: <B>Cat. No. SAB1411651</B>: Antibody concentration: 1-5 μg/mL. Specific band of ~156.4 kDa using HeLa lysate.

ZFYVE9 antibody - orb77801 from Biorbyt

Prices $425.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Western blot analysis of fetal liver,heart and lung lysate using ZFYVE9 antibody

Rabbit anti-human zinc finger, FYVE domain containing 9 (OACA02353) - OACA02353 from Aviva Systems Biology

Prices $425.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

RTN1 antibody - 70R-6965 from Fitzgerald

Prices $345.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

ZFYVE9 antibody - 70R-21384 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

ZFYVE9 Antibody - abx038063 from Abbexa

Host Rabbit
Clonality Polyclonal

Zinc Finger, Fyve Domain Containing 9 Antibody - abx116760 from Abbexa

Host Rabbit
Clonality Polyclonal

ZFYVE9 antibody - 14821-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
Mouse brain were subjected to SDS PAGE followed by western blot with 14821-1-AP(ZFYVE9 Antibody) at dilution of 1:1000

ZFYVE9 antibody - 22033-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical of paraffin-embedded human kidney using 22033-1-AP(ZFYVE9 antibody) at dilution of 1:50 (under 10x lens)

ZFYVE9 antibody - H00009372-D01 from Acris Antibodies

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ZFYVE9 antibody - H00009372-D01P from Acris Antibodies

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

SARA antibody - 22033-1-AP from Proteintech Group

Prices $279.00
Sizes con: 64 μg/150μl
Host Rabbit
Clonality Polyclonal
mouse brain tissue were subjected to SDS PAGE followed by western blot with 22033-1-AP(ZFYVE9 antibody) at dilution of 1:1000

SARA antibody - 14821-1-AP from Proteintech Group

Prices $279.00
Sizes con: 60 μg/150μl
Host Rabbit
Clonality Polyclonal
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14821-1-AP(ZFYVE9 antibody) at dilution of 1:1000
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