Anti-RBPJK antibody - ChIP Grade
| Name | Anti-RBPJK antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab25949 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP ChIP EMSA WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human RBPJK |
| Description | Rabbit Polyclonal |
| Gene | RBPJ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![RBPJK was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to RBPJK and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab25949.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 56kDa, non specific bands -50kDa: This extra band is heavy chain contamination; RBPJK](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/21622_ab25949-212666-IPVI005ab2594920m.jpg)
RBPJK was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to RBPJK and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab25949.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 56kDa, non specific bands -50kDa: This extra band is heavy chain contamination; RBPJK
All lanes : Anti-RBPJK antibody - ChIP Grade (ab25949) at 1/500 dilutionLane 1 : Mouse myoblast C2C12 whole cell lysate at 50 µgLane 2 : Mouse myoblast C2C12 whole cell lysate at 75 µgSecondaryHRP-conjugated Goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Anti-RBPJK antibody - ChIP Grade (ab25949) at 1 µg/ml + Pancreas (Human) Tissue Lysate at 20 µgSecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.
Product References
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Kumar V, Palermo R, Talora C, Campese AF, Checquolo S, Bellavia D, Tottone L, Testa G, Miele E, Indraccolo S, Amadori A, Ferretti E, Gulino A, Vacca A, Screpanti I. Leukemia. 2014 Dec;28(12):2324-35.
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Zhang Y, Lam O, Nguyen MT, Ng G, Pear WS, Ai W, Wang IJ, Kao WW, Liu CY. Development. 2013 Feb 1;140(3):594-605.
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Larabee JL, Shakir SM, Barua S, Ballard JD. J Biol Chem. 2013 Jul 26;288(30):21526-36.
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Bartels SJ, Spruijt CG, Brinkman AB, Jansen PW, Vermeulen M, Stunnenberg HG. PLoS One. 2011;6(10):e25884.
Epigenetic analysis of KSHV latent and lytic genomes. - Epigenetic analysis of KSHV latent and lytic genomes.
Toth Z, Maglinte DT, Lee SH, Lee HR, Wong LY, Brulois KF, Lee S, Buckley JD, Laird PW, Marquez VE, Jung JU. PLoS Pathog. 2010 Jul 22;6(7):e1001013.