Anti-REA antibody [EPR14522] (ab181838) at 1/10000 dilution + HeLa cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-REA antibody [EPR14522] (ab181838) at 1/10000 dilutionLane 1 : Human fetal kidney lysateLane 2 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunofluorescent analysis of acetone-fixed HeLa cells labeling REA with ab181838 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution.
Immunofluorescent analysis of acetone-fixed 293T cells labeling REA with ab181838 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.