All lanes : Anti-REA antibody [EPR14523] (ab182139) at 1/20000 dilutionLane 1 : HT-1080 cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder tissue sections labeling REA using ab182139 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Immunohistochemical analysis of paraffin embedded mouse kidney tissue sections labeling REA using ab182139 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Immunofluorescent analysis of acetone fixed HeLa cells labeling REA using ab182139 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution. Counterstain DAPI.
Immunofluorescent analysis of paraformaldehyde fixed 293T cells labeling REA using ab182139 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution. Counterstain DAPI.