
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RECQL5 with ab91422 at 1/1000 (1µg/ml). Detection: DAB.

All lanes : Anti-RECQL5 antibody (ab91422) at 0.1 µg/mlLane 1 : HeLa cell lysate at 50 µgLane 2 : HeLa cell lysate at 15 µgLane 3 : HeLa cell lysate at 5 µg

SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91422. 20% of the immunoprecipitate was loaded per lane, and probed with ab91422 at 1µg/ml (lane 1) or a control IgG (lane2). Detection: chemoluminescence with an exposure time of 10 seconds.