Anti-RFP antibody

• Supplier: Abcam
• Catalog: ab62341
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF IHC-P SDS-PAGE IHC-F WB IP ICC/IF ICC/IF
• Antigen: Recombinant RFP expressed in E
• Description: Rabbit Polyclonal
• Gene: TRIM27
• Conjugate: Unconjugated

Product images

ab62341, at 1/100, staining chicken midbrain cells by Immunocytochemistry/ Immunofluorescence.The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody for 3 hours. An Alexa fluor® 568 goat polyclonal to rabbit Ig, diluted 1/300, was used as the secondary antibody.See Abreview

All lanes : Anti-RFP antibody (ab62341) at 1/2000 dilutionLane 1 : Whole cell lysate prepared from HEK293 cellsLane 2 : Whole cell lysate prepared from HEK293 cellsLysates/proteins at 20 µg per lane.SecondaryHRP conjugated anti-rabbit at 1/5000 dilutiondeveloped using the ECL technique

ab62341 staining RFP in rat oligodendrocytes transfected with recombinant human adenovirus type 5 expressing Red Fluorescent Protein (RFP) by Immunocytochemistry/ Immunofluorescence.Cells were fixed with formaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 5% serum for 10 minutes at 25°C. Samples were then incubated with ab62341 at a 1/300 dilution for 1 hour at 25°C. The secondary used was an Alexa Fluor® 594 goat anti-rabbit IgG (H+L), used at a 1/500 dilution.See Abreview

Product References

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