TRIM27

Gene Symbol TRIM27
Entrez Gene 5987
Alt Symbol RFP, RNF76
Species Human
Gene Type protein-coding
Description tripartite motif containing 27
Other Description RFP transforming protein|RING finger protein 76|ret finger protein|tripartite motif protein TRIM27|tripartite motif-containing 27|tripartite motif-containing protein 27|zinc finger protein RFP
Swissprots P14373 Q9BZY6 Q5ST26 A2BE15 Q9UJL3 Q6NXR9 Q5RJA8 Q6LA73
Accessions AAF32265 CAB06480 CAI19959 EAX03176 EAX03177 P14373 AB209885 BAD93122 AF230393 AAG50172 AF230394 AAG50173 AJ420519 AX776009 CAE11704 AX776011 CAE11705 BC013580 AAH13580 BC066924 AAH66924 DQ890982 ABM81908 DQ894156 ABM85082 J03407 AAA36564 NM_006510 NP_006501 NM_030950
Function E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T- cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double- stranded DNA. {ECO:0000269|PubMed:10976108, ECO:0000269|PubMed:12807881, ECO:0000269|PubMed:22128329, ECO:0000269|PubMed:23452853}.
Subcellular Location Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. Nucleus, PML body {ECO:0000250}. Early endosome {ECO:0000269|PubMed:1437549, ECO:0000269|PubMed:23452853, ECO:0000269|PubMed:9247190}. Note=Nuclear or cytoplasmic depending on the cell type (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies. Recruited to retromer-containing endosomes via interaction with MAGEL2 (PubMed:23452853). {ECO:0000250, ECO:0000269|PubMed:23452853}.
Tissue Specificity Expressed in testis namely within the seminiferous tubules. {ECO:0000269|PubMed:12445133}.

TRIM27 Antibody - 12663 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using TRIM27 Antibody.

TRIM27 (C-18) - sc-47513 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-RFP antibody - ab62341 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab62341, at 1/100, staining chicken midbrain cells by Immunocytochemistry/ Immunofluorescence.The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody for 3 hours. An Alexa fluor® 568 goat polyclonal to rabbit Ig, diluted 1/300, was used as the secondary antibody.See Abreview

Anti-RFP antibody - ab28664 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RFP antibody - ab152123 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RFP antibody (ab152123) at 1/10000 dilutionLane 1 : Non-transfected 293T whole cell lysateLane 2 : GFP-transfected 293T whole cell lysateLane 3 : RFP-transfected 293T whole cell lysateLysates/proteins at 5 µg per lane.

Anti-RFP antibody - ab124754 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RFP antibody - ab40740 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-RFP antibody (ab40740) at 1 µg/ml + RFP full length protein (ab51993) at 0.1 µgSecondaryGoat Polyclonal to rabbit IgG - H&L - Pre Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-RFP antibody [25] - ab65856 from Abcam

Prices $382.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 25
Anti-RFP antibody [25] (ab65856) at 1/1000 dilution + recombinant RFP protein

Anti-RFP antibody (Biotin) - ab34771 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RFP antibody (Biotin) (ab34771) at 1/2500 dilutionLane 1 : 10 µg of HeLa cell extract containing GFP recombinant proteinLane 2 : 10 µg of a HeLa lysate containing RFP recombinant protein. SecondaryIRDye™800 conjugated Goat anti-Rabbit IgG at 1/5000 dilutionObserved band size : 27 kDa (why is the actual band size different from the predicted?)

Anti-RFP antibody (FITC) - ab34764 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RFP antibody [GT1610] - ab184195 from Abcam

Host Mouse
Clonality Monoclonal
Clone GT1610
All lanes : ab184195 at 1/1000 dilutionLane 1 : 30 μg transfected HEK293T cell lysateLane 2 : Control with 2 μg of preimmune mouse IgGLane 3 : Immunoprecipitation of RFP-fused protein from 400 µg RFP-fused gene transfected HEK293T cell lysate using 2 μg of ab18419512% SDS-PAGE

Anti-RFP antibody (HRP) - ab34767 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RFP antibody [RF5R] - ab125244 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone RF5R
All lanes : Anti-RFP antibody [RF5R] (ab125244) at 1/1000 dilutionLane 1 : HEK293 cell lysate, non-transfectedLane 2 : HEK293 cell lysate, Turbo-RFP-transfectedLane 3 : HEK293 cell lysate, DeRed-transfected developed using the ECL technique

Anti-RFP antibody [RFP.H8] - ab109809 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone RFP.H8
All lanes : Anti-RFP antibody [RFP.H8] (ab109809) at 1/1000 dilutionLane 1 : 293 cell lysate with DsRed Expression at 1 µgLane 2 : 293 cell lysate with DsRed Expression at 5 µgLane 3 : 293 cell lysate with DsRed Expression at 10 µg

Anti-RFP / Red Fluorescent Protein Antibody (N-Terminus, clone RF5R) - LS-C153602 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone RF5R

Anti-RFP / Red Fluorescent Protein Antibody (N-Terminus) - LS-C131815 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-RFP / Red Fluorescent Protein Antibody (HRP) - LS-C154272 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RFP / Red Fluorescent Protein Antibody (FITC) - LS-C154267 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RFP / Red Fluorescent Protein Antibody - LS-C153868 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
Western blot of Anti-RFP Antibody. 0.1 ug of RFP was loaded on a 4-20% gel and transferred to nitrocellulose membrane. Anti-RFP Antibody was added at 1.0 g/mL at RT for 2 hours. IRDye800 anti-Chicken (p/n 603-132-126) was added at 1:20000 at RT for 45 minutes.

Anti-RFP / Red Fluorescent Protein Antibody - LS-C147085 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of RFP / Red Fluorescent Protein antibody LS-C147085.

Anti-RFP / Red Fluorescent Protein Antibody - LS-C60076 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-RFP Antibody - Western Blot. Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kD band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2500 for 1 h at RT followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Goat-a-Rabbit IgG [H&L] MX (. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Anti-RFP / Red Fluorescent Protein Antibody (Biotin) - LS-C154289 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-RFP Antibody - Western Blot Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein. Separation was achieved using a 4-12% Bis-Tris gradient gel followed by transfer to nitrocellulose and blocking. The membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Goat-a-Rabbit IgG [H&L] MX (611-132-122). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Anti-RFP / Red Fluorescent Protein Antibody (C-Terminus) - LS-C182422 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-RFP / Red Fluorescent Protein Antibody - LS-C183456 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RFP / Red Fluorescent Protein Antibody (clone 3G5) - LS-C179854 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3G5
Search more