Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 mES cells and either 5 μl of CHD1 (D8C2) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Mouse Oct-4 Promoter Primers #4653, SimpleChIP ® Mouse RPL30 Intron 2 Primers #7015, and SimpleChIP ® Mouse MYT-1 Promoter Primers #8985. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.