Anti-RhoA antibody [1B12]
| Name | Anti-RhoA antibody [1B12] |
|---|---|
| Supplier | Abcam |
| Catalog | ab54835 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 1B12 |
| Applications | WB IHC-P FC ICC/IF ICC/IF ELISA |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-194 of Human RhoA |
| Description | Mouse Monoclonal |
| Gene | RHOA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab54835 at 10 ug/ml staining RhoA in human Hela cells (ab150035) by Immunocytochemistry / Immunofluorescence.
ICC/IF image of ab54835 stained HeLa cells (ab150035). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54835, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLa cells (ab150035) stained with ab54835 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54835, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/22861_RhoA-Primary-antibodies-ab54835-4.jpg)
Overlay histogram showing HeLa cells (ab150035) stained with ab54835 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54835, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
RhoA antibody (ab54835) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lymphoma tissue.
Product References
Human beta-defensin-1 suppresses tumor migration and invasion and is an - Human beta-defensin-1 suppresses tumor migration and invasion and is an
Han Q, Wang R, Sun C, Jin X, Liu D, Zhao X, Wang L, Ji N, Li J, Zhou Y, Ye L, Liang X, Jiang L, Liao G, Dan H, Zeng X, Chen Q. PLoS One. 2014 Mar 21;9(3):e91867.
Androgen receptor-target genes in african american prostate cancer disparities. - Androgen receptor-target genes in african american prostate cancer disparities.
Wang BD, Yang Q, Ceniccola K, Bianco F, Andrawis R, Jarrett T, Frazier H, Patierno SR, Lee NH. Prostate Cancer. 2013;2013:763569.
Dissecting the inter-substrate navigation of migrating glioblastoma cells with - Dissecting the inter-substrate navigation of migrating glioblastoma cells with
Mertsch S, Oellers P, Wendling M, Stracke W, Thanos S. Mol Neurobiol. 2013 Aug;48(1):169-79.
Notch1 intracellular domain increases cytoplasmic EZH2 levels during early - Notch1 intracellular domain increases cytoplasmic EZH2 levels during early
Roy A, Basak NP, Banerjee S. Cell Death Dis. 2012 Aug 23;3:e380.
Folliculin interacts with p0071 (plakophilin-4) and deficiency is associated with - Folliculin interacts with p0071 (plakophilin-4) and deficiency is associated with
Nahorski MS, Seabra L, Straatman-Iwanowska A, Wingenfeld A, Reiman A, Lu X, Klomp JA, Teh BT, Hatzfeld M, Gissen P, Maher ER. Hum Mol Genet. 2012 Dec 15;21(24):5268-79.
Proteomic identification of RhoA as a potential biomarker for proliferation and - Proteomic identification of RhoA as a potential biomarker for proliferation and
Gou L, Wang W, Tong A, Yao Y, Zhou Y, Yi C, Yang J. J Mol Med (Berl). 2011 Aug;89(8):817-27.
Concurrent suppression of integrin alpha5, radixin, and RhoA phenocopies the - Concurrent suppression of integrin alpha5, radixin, and RhoA phenocopies the
Valastyan S, Chang A, Benaich N, Reinhardt F, Weinberg RA. Cancer Res. 2010 Jun 15;70(12):5147-54.
A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis. - A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis.
Valastyan S, Reinhardt F, Benaich N, Calogrias D, Szasz AM, Wang ZC, Brock JE, Richardson AL, Weinberg RA. Cell. 2009 Jun 12;137(6):1032-46.
RSK1 drives p27Kip1 phosphorylation at T198 to promote RhoA inhibition and - RSK1 drives p27Kip1 phosphorylation at T198 to promote RhoA inhibition and
Larrea MD, Hong F, Wander SA, da Silva TG, Helfman D, Lannigan D, Smith JA, Slingerland JM. Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9268-73. doi:
Rho GTPases modulate entry of Ebola virus and vesicular stomatitis virus - Rho GTPases modulate entry of Ebola virus and vesicular stomatitis virus
Quinn K, Brindley MA, Weller ML, Kaludov N, Kondratowicz A, Hunt CL, Sinn PL, McCray PB Jr, Stein CS, Davidson BL, Flick R, Mandell R, Staplin W, Maury W, Chiorini JA. J Virol. 2009 Oct;83(19):10176-86.