ChIP analysis of
c-
Myc promoter occupancy in response to lithium stimulation in serum synchronized C2C12 cells. Antibodies tested include HDAC1 (H-15): sc-7872, HDAC1 (C-19): sc-6298 and HDAC1 (H-11): sc-8410 (A), β-catenin (H-120): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (B), E2F-4 (A-20): sc-1082, E2F-4 (D-3): sc-6851, E2F-4 (RK-13): sc-511 and E2F-4 (C-20): sc-866 (C), and p130 (C-20): sc-317, p130 (211.6): sc-9963 and p130 (H-125): sc-20678 (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA.
ChIP analysis of transcription regulatory proteins associated with the Pitx2 promoter in aT3-1 cells in response to lithium. Antibodies tested include LEF-1 (C-19): sc-8592 amd LEF-1 (N-17): sc-8591 (B), β-catenin (H-102): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (C), HDAC1 (H-11): sc-7872, HDAC1 (C-19): sc-6298 and HDAC1 (H-11): sc-8410 (D), HDAC2 (C-8): sc-9959, HDAC2 (H-54): sc-7899 and HDAC2 (C-19): sc-6296 (E). Input control (A). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.
HDAC1 (H-11) PE: sc-8410 PE. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-2866.
HDAC1 (H-11): sc-8410. Western blot analysis of HDAC1 expression in K-562 nuclear extract.
HDAC1 (H-11): sc-8410. Western blot analysis of HDAC1 expression in HeLa (A), Jurkat (B), K-562 (C) and NIH/3T3 (D) whole cell lysates.