Anti-RHOC antibody
| Name | Anti-RHOC antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab64659 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Zebrafish, Zebra finch |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human RHOC |
| Description | Rabbit Polyclonal |
| Gene | RHOC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-RHOC antibody (ab64659) at 1 µg/mlLane 1 : EBS E14 TG2A Day 3 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell LysateLane 2 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab64659 stained HeLa cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64659, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.
Product References
The SmgGDS splice variant SmgGDS-558 is a key promoter of tumor growth and RhoA - The SmgGDS splice variant SmgGDS-558 is a key promoter of tumor growth and RhoA
Hauser AD, Bergom C, Schuld NJ, Chen X, Lorimer EL, Huang J, Mackinnon AC, Williams CL. Mol Cancer Res. 2014 Jan;12(1):130-42.
Human beta-defensin-1 suppresses tumor migration and invasion and is an - Human beta-defensin-1 suppresses tumor migration and invasion and is an
Han Q, Wang R, Sun C, Jin X, Liu D, Zhao X, Wang L, Ji N, Li J, Zhou Y, Ye L, Liang X, Jiang L, Liao G, Dan H, Zeng X, Chen Q. PLoS One. 2014 Mar 21;9(3):e91867.